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mouse geckov2 library b  (Addgene inc)


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    Structured Review

    Addgene inc mouse geckov2 library b
    Mouse Geckov2 Library B, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+geckov2+crispr+knockout+pooled+library+b/Mouse+CRISPR+Knockout+Pooled+Library+(GeCKO+v2)+(Pooled+Library+%231000000052%2C+%231000000053)/pmc12041282-237-1-4
    Average 93 stars, based on 38 article reviews
    mouse geckov2 library b - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation.
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. Autophagy loss of function screen MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab39. .. Infected cells were selected with puromycin (2μg/mL, Millipore) for 8 days.

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9-Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab . .. Infected cells were selected with puromycin (2 μg/mL, Millipore) for 8 days.

    Expressing:

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation.
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. Autophagy loss of function screen MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab39. .. Infected cells were selected with puromycin (2μg/mL, Millipore) for 8 days.

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9-Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab . .. Infected cells were selected with puromycin (2 μg/mL, Millipore) for 8 days.

    Plasmid Preparation:

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation.
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. Autophagy loss of function screen MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab39. .. Infected cells were selected with puromycin (2μg/mL, Millipore) for 8 days.

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9-Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab . .. Infected cells were selected with puromycin (2 μg/mL, Millipore) for 8 days.

    Transduction:

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation.
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. Autophagy loss of function screen MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab39. .. Infected cells were selected with puromycin (2μg/mL, Millipore) for 8 days.

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9-Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab . .. Infected cells were selected with puromycin (2 μg/mL, Millipore) for 8 days.

    CRISPR:

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation.
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. Autophagy loss of function screen MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab39. .. Infected cells were selected with puromycin (2μg/mL, Millipore) for 8 days.

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9-Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab . .. Infected cells were selected with puromycin (2 μg/mL, Millipore) for 8 days.

    Knock-Out:

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation.
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. Autophagy loss of function screen MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab39. .. Infected cells were selected with puromycin (2μg/mL, Millipore) for 8 days.

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9-Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab . .. Infected cells were selected with puromycin (2 μg/mL, Millipore) for 8 days.

    Purification:

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation.
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. Autophagy loss of function screen MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab39. .. Infected cells were selected with puromycin (2μg/mL, Millipore) for 8 days.

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9-Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab . .. Infected cells were selected with puromycin (2 μg/mL, Millipore) for 8 days.

    Virus:

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation.
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. Autophagy loss of function screen MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab39. .. Infected cells were selected with puromycin (2μg/mL, Millipore) for 8 days.

    Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation
    Article Snippet: Custom antibodies raised against RNAseK were produced by Proteintech using the following antigen peptide sequence: CQVRLNKRKEYMVR (WB 1:2000, IF 1:600). .. MEF cells stably expressing pBabe-GFP-LC3 and SpCas9 (lentiCas9-Blast, a gift from Feng Zhang, Addgene plasmid #52962) was transduced with the mouse GeCKOv2 CRISPR knockout pooled library B (a gift from Feng Zhang, Addgene #1000000053), where purified virus was provided by Kevin Myant’s lab . .. Infected cells were selected with puromycin (2 μg/mL, Millipore) for 8 days.



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